High-resolution custom array spanning short arm of chromosome 17. Homo sapiens

The goal of this experiment was to determine the size, genomic extent and gene content of rearrangements involving 17p. Overall design: To determine size, genomic extent and gene content for each rearrangement, we used a customized tiling-path oligonucleotide microarray spanning the entire 17p chromosomal region. A 4x44k Agilent Technologies (Santa Clara, CA) microarray was designed using the Agilent e-array website. We selected 42,000 probes covering Chr17: 29,000-22,100,000 (hg18), which represents an average distribution of 1 probe per 526 bp.

本实验旨在明确涉及17号染色体短臂（17p）的染色体重排的大小、基因组覆盖范围及基因构成。实验总体设计：为明确每一例染色体重排的大小、基因组覆盖范围与基因构成，我们采用了覆盖完整17p染色体区域的定制化平铺路径寡核苷酸微阵列（tiling-path oligonucleotide microarray）。本次实验使用的4x44k安捷伦科技（Agilent Technologies，美国加利福尼亚州圣克拉拉市）微阵列，通过安捷伦e-array平台设计完成。我们选取了覆盖人类17号染色体Chr17: 29,000-22,100,000（hg18参考基因组版本）的42,000个探针，平均每526个碱基对分布1个探针。