PCR primers used to amplify cDNA sequences of both TcAce1 and TcAce2 genes and to analyze their gene expressions.

aProduct location refers to the PCR fragment corresponding to the Ace gene nucleotide sequence of T. castaneum from NCBI database (TcAce1: XM_968369; TcAce2: XM_965681). N/A refers to the sequence based on the genomic sequence in Beetlebase (http://beetlebase.org/).bF and R refer to forward and reverse primers, respectively.

a. 产物位置指的是源自NCBI数据库的赤拟谷盗（Tribolium castaneum，T. castaneum）Ace基因核苷酸序列对应的聚合酶链式反应（PCR）片段，其中TcAce1对应登录号XM_968369，TcAce2对应登录号XM_965681。N/A指代基于Beetlebase数据库（http://beetlebase.org/）的基因组序列得到的序列。
b. F与R分别指代正向引物与反向引物。