Proteomic profiling of ectodermal basal disc cells secretion of Hydra

Biological adhesion (bioadhesion) is referred to attachment of organisms to either biotic or abiotic surfaces. The differentiated ectodermal basal disc cells of the freshwater cnidarian Hydra secrete proteinaceous glue to temporarily attach themselves to surfaces underwater. In this study, we investigate for the first time the protein content of adhesive secretions from the freshwater cnidarian Hydra magnipapillata strain 105. This secretome were analysed using mass spectrometry and resulting MS/MS data were searched against in silico translated H. magnipapillata transcriptome and results from gene expression.

生物粘附（biological adhesion，bioadhesion）指生物体附着于生物或非生物表面的现象。淡水刺胞动物水螅（Hydra）已分化的外胚层基盘细胞会分泌蛋白类胶黏物质，以暂时将自身固定于水下表面。本研究首次针对淡水刺胞动物大乳头水螅（Hydra magnipapillata）105品系的粘附分泌物的蛋白组分展开研究。本研究采用质谱分析法（mass spectrometry）对该分泌组（secretome）进行解析，并将获取的串联质谱（MS/MS）数据与经计算机模拟（in silico）翻译的大乳头水螅转录组（transcriptome）以及基因表达（gene expression）分析结果进行比对检索。