Oleic Acid Induces Tissue-Resident FOXP3 Regulatory T Cell Suppressive Functions (single-cell RNA)

Comparing the transcriptomic program induced by oleic acid to that of the pro-inflammatory arachidonic acid, we find that Tregs sorted from peripheral blood and adipose of healthy donors transcriptomically resemble the oleic acid in vitro treated Tregs, whereas Tregs obtained from the adipose tissue of relapsing-remitting MS patients more closely resemble an arachidonic acid profile Peripheral blood and adipose tissue were collected from eight healthy donors and eight multiple sclerosis (MS) patients. Two of the MS patients were untreated (> ~1.5yrs since last treatment at the time of the procedure), and the rest had previously received disease modifying treatments (DMTs) (> 6 months prior to the procedure, but off treatment at the time of the procedure). Height and weight (and therefore BMI) are self-reported. Donor group abbreviations: HC = healthy conrols, MS.NoTrt and MS.PrevTrt = MS patients, untreated or previously treated, respectively.

本研究将油酸诱导的转录组程序（transcriptomic program）与促炎性花生四烯酸诱导的转录组程序进行对比，结果发现：从健康供者外周血与脂肪组织分选获得的调节性T细胞（Tregs），其转录组特征与体外经油酸处理的Tregs相似；而从复发缓解型多发性硬化（relapsing-remitting multiple sclerosis, MS）患者脂肪组织中分离的Tregs，则更接近于花生四烯酸处理后的转录组特征。本研究共收集8名健康供者与8名多发性硬化（MS）患者的外周血及脂肪组织样本。其中2名MS患者未接受过疾病修饰治疗（disease modifying treatments, DMTs）（距上次治疗至样本采集时已超过约1.5年），其余患者此前均接受过疾病修饰治疗，且在样本采集前已停药超过6个月。供者的身高、体重（据此计算体质量指数BMI）均为自我报告。供体分组缩写说明：HC代表健康对照（healthy controls），MS.NoTrt与MS.PrevTrt分别代表未接受治疗与既往接受治疗的MS患者。