Pond water SIP fraction microbial communities from Walnut Creek, CA, United States - JGIPond_B_20pct_30ng_1_1.6855 metagenome

SIP metagenome fraction of pond sample from Joint Genome Institute, Walnut Creek spiked with Pseudomonas putida & Escherichia coli bacterial isolates (for each isolate 30ng of DNA labeled at 20pct with C13 were added); replicate number 1. This fraction has a SIP fraction number 18 and DNA density of 1.6855 g/uL. This is part of a larger study to identify the limit of detection of JGI SIP metagenomics pipeline.

本样本为来自核桃溪联合基因组研究所（Joint Genome Institute, JGI）的池塘样本的稳定同位素探针（Stable Isotope Probing, SIP）宏基因组组分，其中掺入了恶臭假单胞菌（Pseudomonas putida）与大肠杆菌（Escherichia coli）的细菌分离株：针对每株分离株，均添加了20%碳13（C¹³）标记的DNA，添加量为30纳克，该样本为第1次重复实验样本。该组分的SIP组分编号为18，DNA密度为1.6855 g/μL。本样本属于一项旨在确定JGI SIP宏基因组分析流程检测限的大型研究的组成部分。