Combining TSS-MPRA and sensitive TSS profile dissimilarity scoring to study the sequence determinants of transcription initiation

Using a transcription start site-capturing massively parallel reporter assay (TSS-MPRA) that simultaneously measures the location and frequency of transcription initiation from synthetic sequences, we carefully characterize the degree to which plasmid-based MPRAs recapitulate endogenous initiation patterns. Overall design: TSS-MPRA sequencing of insert libraries to characterize transcription initiation patterns (K562)

本研究采用转录起始位点捕获型大规模并行报告基因检测（transcription start site-capturing massively parallel reporter assay, TSS-MPRA）技术，该技术可同时检测合成序列中转录起始的位置与发生频率，借此系统表征了基于质粒的大规模并行报告基因检测（massively parallel reporter assay, MPRA）重现内源转录起始模式的程度。实验设计概述：通过对插入片段文库开展TSS-MPRA测序，以表征K562细胞系中的转录起始模式。