Gene-expression profiles of multi-stage hepatocellular carcinoma induced by diethylnitrosamine (DEN) in mouse model. Mus musculus

To better characterize the pathogenesis of hepatocarcinogenesis, we established mouse HCC model by diethylnitrosamine (DEN) exposures and performed whole genome expression profiling. Overall design: Total was extracted from frozen liver samples from vehicle-control and DEN treated mice. Histologic classification of adenomas (n=7), eHCCs (n=11), and aHCCs (n=7) was performed.

为更清晰地阐明肝细胞癌变（hepatocarcinogenesis）的发病机制，我们通过二乙基亚硝胺（diethylnitrosamine, DEN）处理构建了小鼠肝细胞癌（hepatocellular carcinoma, HCC）模型，并开展了全基因组表达谱分析。总体实验设计：从溶剂对照组及二乙基亚硝胺处理小鼠的冰冻肝脏样本中提取总RNA。本研究完成了腺瘤（adenomas, n=7）、早期肝细胞癌（early HCC, eHCC, n=11）及进展期肝细胞癌（advanced HCC, aHCC, n=7）的组织学分类。