Material and method for resequencing.

Agarwood is the resinous part of the injured tree of the Aquilaria species of the Thymelaeceae family, Qi-Nan is a type of agarwood that forms easily and benefits from a shortened inducing time, noted for features like its substantial resin, (2-phenylethyl) chromone and 2-[2-(4-methoxyphenyl) ethyl] chromone content. Although the content of agarotetrol in Qi-Nan is very low, it is still an important indicator for the agarwood quality. A validated method was developed to precisely quantify both agarotetrol and two aforementioned chromones, as well as ethanal extracts, within Qi-Nan to explore how the inducing time (half a year, one year, and two years) and different clones (YYZ, AS, RH, XGY) affect agarwood quality. Different clones and inducing time both have significant effects on agarwood quality. The agarwood quality of Qi-Nan does not necessarily improve with a longer inducing time. YYZ, AS, and RH clones reached to the best agarwood quality after two years of induction. The ethanol extracts content was among 46.82 ~ 48.59%, two chromones were among 10.57% ~ 14.05% after two years of induction. However, for XGY clone, the quality of agarwood after one year of induction was better than that after two years, which reached to the highest ethanol extracts (51.49%) and two chromones content reached to 12.43% and 12.91% after one year of induction. Therefore, for different clones of Qi-Nan, harvesting should be conducted at different time of induction for agarwood formations, which has theoretical implications for the cultivation and industrial development of cultivated Qi-Nan agarwood.

沉香是瑞香科（Thymelaeceae）沉香属（Aquilaria）植物受损伤后形成的含树脂部分。奇楠（Qi-Nan）是一类易于形成且诱导周期更短的沉香，以其高树脂含量、(2-苯乙基)色酮（(2-phenylethyl) chromone）与2-[2-(4-甲氧基苯基)乙基]色酮（2-[2-(4-methoxyphenyl) ethyl] chromone）的含量为核心特征。尽管奇楠中的沉香四醇（agarotetrol）含量极低，但它仍是评价沉香品质的重要指标。本研究开发了一种经过验证的分析方法，可精准定量奇楠中的沉香四醇、上述两种色酮以及乙醇提取物含量，以探究诱导时长（半年、1年、2年）与不同无性系（YYZ、AS、RH、XGY）对沉香品质的影响。不同无性系与诱导时长均对沉香品质存在显著影响。奇楠的沉香品质未必随诱导时长延长而提升。YYZ、AS及RH无性系在诱导2年后达到最优沉香品质，此时其乙醇提取物含量介于46.82%~48.59%，两种色酮的含量区间为10.57%~14.05%。然而对于XGY无性系，诱导1年后的沉香品质优于诱导2年后：其乙醇提取物含量达最高值51.49%，两种色酮含量分别为12.43%与12.91%。因此，针对不同奇楠无性系，应在适配的诱导时长节点进行采收以获取优质沉香，该研究结果对人工种植奇楠沉香的栽培与产业开发具有重要的理论指导意义。