Table_8_RNA-Seq Reveals miRNA and mRNA Co-regulate Muscle Differentiation in Fetal Leizhou Goats.XLS

Muscle differentiation is an essential link in animal growth and development, and microRNA and mRNA are indispensable in skeletal muscle differentiation. To improve the meat quality and production of the Leizhou goat, it is vital to understand the molecular mechanism by which its skeletal muscle differentiates. By RNA sequencing (RNA-SEQ), we established miRNA-mRNA profiles of Leizhou goats at three stages: fetal day 70, 90, and 120. There were 991 differently expressed mRNAs and 39 differentially expressed miRNAs found, with the differentially expressed mRNAs mainly enriched in calcium ion binding, ECM-receptor interaction, and Focal adhesion. CKM and MYH3, two muscle differentiation markers, were significantly differentially expressed during this period. In addition, we found that chi-miR-129-5p, chi-miR-433, and chi-miR-24-3p co-regulate muscle differentiation with their target genes. Finally, we can confirm that muscle differentiation occurred in Leizhou goat between 90 and 120 days of the fetus. This study is helpful to better explore the molecular mechanism of goat muscle differentiation.

肌肉分化是动物生长发育过程中的关键环节，而微小RNA（microRNA）与信使RNA（mRNA）在骨骼肌分化过程中发挥不可或缺的作用。为提升雷州山羊的肉品质与生产性能，解析其骨骼肌分化的分子机制至关重要。本研究通过RNA测序（RNA-seq）技术，构建了雷州山羊三个胎龄阶段（妊娠第70、90、120天）的miRNA-mRNA联合表达谱。研究共鉴定出991个差异表达mRNA与39个差异表达miRNA，差异表达mRNA主要富集于钙离子结合、细胞外基质-受体相互作用以及黏着斑通路。肌分化标志物CKM与MYH3在此阶段均呈现显著差异表达。此外，本研究发现chi-miR-129-5p、chi-miR-433以及chi-miR-24-3p可通过靶向调控靶基因协同参与肌肉分化过程。最终证实雷州山羊的肌肉分化发生于胎儿妊娠第90至120天期间。本研究可为深入解析山羊肌肉分化的分子机制提供重要参考。