Functional profiling and visualization of the sphingolipid metabolic network in vivo (mCD8::GFP)

This dataset contains a comprehensive collection of z-stack confocal images from whole-mount adult Drosophila melanogaster brains. The images document the detailed neuroanatomical expression patterns of 52 genes essential for sphingolipid metabolism. The expression of each gene was visualized using the GAL4-UAS binary system. Specific GAL4 reporter lines (C-terminus 3XHA-T2A-GAL4 insertion), which faithfully recapitulate the endogenous expression of the target genes, were used to drive a membrane-tethered Green Fluorescent Protein (UAS-mCD8GFP).

本数据集收录了一套全面的成年黑腹果蝇（Drosophila melanogaster）整装大脑的Z轴堆叠共聚焦图像集合。该图像集详细记录了52种鞘脂代谢关键基因的神经解剖学表达模式。所有基因的表达均通过GAL4-UAS二元表达系统（GAL4-UAS binary system）进行可视化：研究采用能够精准重现靶基因内源表达模式的特异性GAL4报告品系（C端3XHA-T2A-GAL4插入位点），驱动膜锚定绿色荧光蛋白（UAS-mCD8GFP）的表达。