Intrapericardial administration of secretomes from endometrial-derived mesenchymal stromal cells: effects on immune-related genes in a porcine model of myocardial infarction

Using a porcine model of acute myocardial infarction, we explored the potential effect of secretomes from endometrial-derived mesenchymal stromal cells (endMSCs) and from IFNγ/TNFα-primed endMSCs on immune-related genes in the infarcted tissue. Infarcted tissues from pigs administered with placebo (n = 4), secretomes from endometrial mesenchymal stromal cells (S-enMSCs) (n = 4), and secretomes from IFNγ/TNFα-primed  endometrial derived  mesenchymal stromal cells (S-enMSCs*) were collected at 7 days post-therapy for RNA extraction and Affymetrix microarrays. Differentially gene expression analysis between biogroups were performed and immuno-related genes were determined.

本研究以急性心肌梗死猪模型为对象，探究子宫内膜来源间充质基质细胞（endometrial-derived mesenchymal stromal cells, endMSCs）及其经干扰素γ（IFNγ）/肿瘤坏死因子α（TNFα）预处理的endMSCs所分泌的分泌组，对梗死组织中免疫相关基因的潜在影响。本研究设置三组实验组：安慰剂给药组（n=4）、子宫内膜间充质基质细胞分泌组（S-enMSCs，n=4）以及经IFNγ/TNFα预处理的子宫内膜来源间充质基质细胞分泌组（S-enMSCs*，n=4）；于治疗后7天采集各组梗死组织，用于RNA提取与Affymetrix基因芯片检测。随后对各组生物组开展差异基因表达分析，并鉴定免疫相关基因的表达情况。