Expression data from Arabidopsis root in response to boron limitation. Arabidopsis thaliana

Boron is essential for plants, and boron availability in soil is an important determinant of agricultural production. Boron availability in soil is limited at many regions in the world, including Japan. Under boron deficient conditions, leaf expansion and root elongation, apical dominance, flower development,and fruit and seed sets are inhibited. In this work, we analyzed the mRNA expression of genes containing AUGUAA motif in their 5′-UTR, which is induced by boron. We used microarrays to detail the global gene expression underlying boron deficiency in roots. Overall design: Plants were grown on solid medium containing 1% (w/v) sucrose, 1.5% (w/v) gellan gum and 100 µM boron for 10 days and then transferred to 0.3 and 100 µM boron for 2 days. Plates were placed vertically at 22°C in a growth chamber under long-day conditions (16 h light/8 h dark cycle). We analyzed the transcript profiles in roots by microarray analysis (Affymetrix ATH1 Genome Array).

硼是植物生长所必需的营养元素，土壤中硼的生物有效性是农业生产的重要决定因子。全球诸多区域（包括日本）的土壤有效硼含量普遍受限。在硼缺乏条件下，植物的叶片扩展、根伸长、顶端优势、花发育以及果实与种子结实过程均会受到抑制。本研究针对5'非翻译区（5'-UTR）携带AUGUAA基序且受硼诱导的基因的信使RNA（mRNA）表达水平展开分析，利用微阵列技术详细解析了根部响应硼缺乏胁迫的全局基因表达谱。实验总体设计：将植物培养于含1%（w/v）蔗糖、1.5%（w/v）结冷胶以及100 µM硼的固体培养基中，培养10天后，分别转移至硼浓度为0.3 µM和100 µM的培养基中继续培养2天。培养板垂直放置于22℃的生长箱内，采用长日照培养条件（16小时光照/8小时黑暗循环）。我们通过微阵列分析（Affymetrix ATH1基因组芯片）对植物根部的转录本表达谱进行了检测。