EASE analysis.

Using EASE (http://david.abcc.ncifcrf.gov/), genes with increased expression in IAD are significantly overrepresented in gene ontology biological process categories that are important for synaptic function such as synaptic transmission, neuronal cell recognition and vesicle-mediated transmission. Genes with decreased expression in IAD are significantly overrepresented in categories relevant to chromatin modification, cell-cycle and carbohydrate metabolism. EASE score equals the upper bound of the distribution of Jacknife Fisher exact probabilities given the List Hits, List Total, Population Hits and Population Total. Upregulated genes (303) and downregulated genes (157) were separately analyzed with EASE.

本研究借助EASE（http://david.abcc.ncifcrf.gov/）工具开展分析，结果显示：在IAD中表达上调的基因，在与突触功能密切相关的基因本体（Gene Ontology）生物过程类别中显著富集，具体包括突触传递、神经元细胞识别以及囊泡介导的传递等过程；而在IAD中表达下调的基因，则显著富集于染色质修饰、细胞周期及碳水化合物代谢相关的功能类别中。EASE分值为给定列表命中基因数（List Hits）、列表总基因数（List Total）、群体命中基因数（Population Hits）以及群体总基因数（Population Total）时，刀切费希尔精确概率（Jacknife Fisher exact probabilities）分布的上界。本研究分别对303个上调基因与157个下调基因使用EASE工具进行了独立分析。