Ribosome profiling of lncRNA-derived sORFs in radiosensitive and radioresistant HNSCCs

Head and Neck Squamous Cell Carcinoma (HNSCC) is a common cause of cancer death. Despite enormous technical advances in surgery and radiotherapy in the recent decade, survival of HNSCC patients has not markedly improved, with only 30% of patients diagnosed with advanced HNSCC that will survive for 5 years. This highlights the need to look into molecular processes leading to mechanisms of HNSCC radioresistance in HNSCC and identify novel radiosensitizers. A growing body of evidence suggests that long non-coding RNAs (lncRNA) containing small open reading frames (sORFs) produce biologically active micropeptides. We performed ribosome profilming of radiosensitive and radioresistant HNSCC cells to identify the lncRNA-encoded micropeptides differentially expressed in radioresistance cellls. Overall design: Ribosome profiling of parental radiosensitive SCC61 and radioresistant RTR61 cells

头颈部鳞状细胞癌（Head and Neck Squamous Cell Carcinoma, HNSCC）是癌症相关死亡的常见诱因。尽管近十年外科与放射治疗技术取得了巨大进步，但头颈部鳞状细胞癌患者的生存率并未得到显著提升，仅30%的晚期头颈部鳞状细胞癌患者可存活5年。这一现状凸显了深入探究介导头颈部鳞状细胞癌放射抵抗的分子过程与机制、筛选新型放射增敏剂的迫切需求。越来越多的研究证据表明，携带小开放阅读框（small open reading frames, sORFs）的长链非编码RNA（long non-coding RNAs, lncRNA）能够编码具有生物学活性的微肽。本研究对放射敏感与放射抵抗的头颈部鳞状细胞癌细胞系开展核糖体谱分析，以筛选在放射抵抗细胞中差异表达的长链非编码RNA编码微肽。总体设计：对亲本放射敏感细胞系SCC61及其放射抵抗衍生细胞系RTR61进行核糖体谱分析。