Isolation, Purification, and Characterization of the PR Oxidase from Penicillium roqueforti

The PR oxidase, an extracellular enzyme, involved in the conversion of PR toxin into PR acid, was purified from the culture broth of Penicillium roqueforti ATCC 48936. The enzyme has a pI of 4.5 and a molecular mass of approximately 88 kDa, and it is a monomer. The optimum pH for this enzyme is ca. 4.0, and the optimum temperature is 50°C.

PR氧化酶（PR oxidase）是一种可催化PR毒素转化为PR酸的胞外酶，该酶从产紫青霉（Penicillium roqueforti）ATCC 48936的培养液中纯化得到。该酶的等电点（pI）为4.5，分子量约为88 kDa，且为单体蛋白；其最适pH约为4.0，最适反应温度为50℃。