RNA profiling of IL-34 overexpressing acute myeloid leukemia (AML) cells

Acute myeloid leukemia (AML) is a highly heterogeneous disease. In our study, we found that overexpressing IL-34 in AML cells accelerates the AML progression and shorten the mice survival, which is medicated by LSC level. We used one of the LSC markers, c-Kit, to divide AML cells. The goals of this study aim to reveal the mechanism mediated by IL-34 overexpression, we performed the RNAseq profiling of MA9-c-kit-, MA9-c-kit+ and MA9-IL-34 AML cells. Overall design: c-kit+ cells were isolated from MA9 and MA9-IL-34 mice. c-kit- cells were isolated from MA9 mice. mRNA profiles of these cells were generated by RNA sequencing.

急性髓系白血病（Acute myeloid leukemia, AML）是一种高度异质性疾病。本研究发现，在AML细胞中过表达IL-34可加速AML进展并缩短小鼠生存期，该效应由白血病干细胞（Leukemia Stem Cell, LSC）水平介导。我们采用LSC标记物之一的c-Kit对AML细胞进行分组。本研究旨在揭示IL-34过表达所介导的作用机制，因此对MA9-c-kit-、MA9-c-kit+及MA9-IL-34三种AML细胞进行了RNA测序（RNAseq）表达谱分析。整体实验设计：从MA9小鼠与MA9-IL-34小鼠体内分离得到c-kit+细胞，从MA9小鼠体内分离得到c-kit-细胞；通过RNA测序获取上述细胞的mRNA表达谱。