Zinc finger DNA-binding conditioned site-specific recombination

Sequence data from "Zinc finger DNA-binding conditioned site-specific recombination" publication. This study contains sequence data for the determination of optimal fusion proteins of recombinases and zinc fingers. Optimal insertional fusion positions were determined with pentapeptide scanning mutageneisis followed by PacBio Hifi or Oxford Nanopore Technologies sequencing. Additionally, a high throughput screen to determine the optimal target site distance, linker composition and zinc finger orientation was performed using nanopore sequencing.

本序列数据集源自《锌指DNA结合型条件性位点特异性重组》（Zinc finger DNA-binding conditioned site-specific recombination）的学术研究。本研究包含用于筛选重组酶与锌指最优融合蛋白的序列数据。研究人员通过五肽扫描诱变（pentapeptide scanning mutagenesis）确定最优插入融合位点，随后采用PacBio HiFi测序或牛津纳米孔科技公司（Oxford Nanopore Technologies）测序完成后续检测。此外，本研究采用纳米孔测序开展高通量筛选，以确定最优的靶点间距、接头组成及锌指取向。