Dataset for: Thermofluidic heat exchangers for actuation of transcription in artificial tissues

Dataset for:

Thermofluidic heat exchangers for actuation of transcription in artificial tissues

Daniel C. Corbett1,2, Wesley B. Fabyan1,2, Bagrat Grigoryan3, Colleen E. O’Connor1,2,
Fredrik Johansson1,2, Ivan Batalov1,2, Mary C. Regier1,2, Cole A. DeForest1,2,4,
Jordan S. Miller3, Kelly R. Stevens1,2,5,6*

1Department of Bioengineering, University of Washington, Seattle, WA 98195, USA. 2Institute for Stem Cell and Regenerative Medicine, Seattle, WA 98195, USA. 3Department
of Bioengineering, Rice University, Houston, TX 77005, USA. 4Department of
Chemical Engineering, University of Washington, Seattle, WA 98195, USA. 5Department
of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195,
USA. 6Brotman Baty Institute, University of Washington, Seattle, WA 98195, USA.
*Corresponding author. Email: [e-mail]

Spatial patterns of gene expression in living organisms orchestrate cell decisions in development, homeostasis, and disease. However, most methods for reconstructing gene patterning in 3D cell culture and artificial tissues are restricted by patterning depth and scale. We introduce a depth- and scale-flexible method to direct volumetric gene expression patterning in 3D artificial tissues, which we call “heat exchangers for actuation of transcription” (HEAT). This approach leverages fluid-based heat transfer from printed networks in the tissues to activate heat-inducible transgenes expressed by embedded cells. We show that gene expression patterning can be tuned both spatially and dynamically by varying channel network architecture, fluid temperature, fluid flow direction, and stimulation timing in a user-defined manner and maintained in vivo. We apply this approach to activate the 3D positional expression of Wnt ligands and Wnt/-catenin pathway regulators, which are major regulators of development, homeostasis, regeneration, and cancer throughout the animal kingdom.

本数据集对应研究：用于人工组织中转录激活的热流体热交换器（Thermofluidic heat exchangers for actuation of transcription in artificial tissues）

作者：Daniel C. Corbett1,2, Wesley B. Fabyan1,2, Bagrat Grigoryan3, Colleen E. O’Connor1,2, Fredrik Johansson1,2, Ivan Batalov1,2, Mary C. Regier1,2, Cole A. DeForest1,2,4, Jordan S. Miller3, Kelly R. Stevens1,2,5,6*

作者单位：
1 华盛顿大学生物工程系，美国华盛顿州西雅图市 98195。2 华盛顿大学干细胞与再生医学研究所，美国华盛顿州西雅图市 98195。3 莱斯大学生物工程系，美国得克萨斯州休斯顿市 77005。4 华盛顿大学化学工程系，美国华盛顿州西雅图市 98195。5 华盛顿大学实验医学与病理学系，美国华盛顿州西雅图市 98195。6 华盛顿大学Brotman Baty研究所，美国华盛顿州西雅图市 98195。

*通讯作者。邮箱：[e-mail]

活体生物中基因表达的空间模式，可调控发育、内稳态与疾病进程中的细胞命运决策。然而，当前多数用于三维细胞培养与人工组织内基因模式重构的方法，均受限于构图深度与尺度范围。本研究提出一种兼具深度与尺度灵活性的方法，可实现三维人工组织内的体积级基因表达模式定向调控，我们将其命名为"转录激活热交换器"（heat exchangers for actuation of transcription，简称HEAT）。该方法依托组织内打印网络的流体热传递，激活嵌入细胞所表达的热诱导转基因。研究证实，通过自定义调整通道网络结构、流体温度、流体流向与刺激时机，可实现基因表达模式的空间与动态调控，且该模式可在活体环境中维持。我们将该方法应用于激活Wnt配体与Wnt/β-连环蛋白通路调控因子的三维位置特异性表达，这类因子是整个动物界中发育、内稳态、再生与癌症的核心调控因子。