Rates of [14C] Glucose in Normal and Diabetic Rat Retina.

Incubation was carried at 37°C for 20 min in the presence of glucose of different concentrations. The activity of PPP is taken as one sixth of CO2 produced from [U-14C] glucose. The results are expressed in nmol/mg protein. Values are mean ± SEM for at least five experiments.*p = 0.02 respect to non diabetic;§ p≤ 0.03 respect 5mM.Rates of [14C] Glucose in Normal and Diabetic Rat Retina.

将反应体系置于37℃、不同浓度葡萄糖的环境中孵育20分钟。以[U-14C]葡萄糖代谢产生的二氧化碳释放量的1/6作为磷酸戊糖途径（pentose phosphate pathway, PPP）的活性表征。实验结果以nmol/mg蛋白为单位进行表述。所有数值均为至少5次独立重复实验的平均值±标准误（standard error of the mean, SEM）。*p=0.02，与非糖尿病组相比；§p≤0.03，与5mM葡萄糖组相比。正常与糖尿病模型大鼠视网膜的[14C]葡萄糖代谢速率