DNA microarray analysis of E.coli K12 MG1655 exposed to prolonged stationary phase

This study explores the effects of prolonged stationary phase, on whole genome expression of Escherichia coli. In summary these results imply that prolonged stationary phase cells have shown less growth rate compared to fresh cells due to the low abundance of transcripts for translation initiation factor infC, and for all the seven rrn operons of 5S, 16S, 23S ribosomal RNA which further delayed in protein synthesis leading to low biomass in prolonged stationary phase cells. 28 days old E.coli cells were grown in the presence of LB broth and LB broth supplemented with 10% v/v glycerol. RNA was extracted from these samples, cDNA was prepared, fragmented cDNA was labled with labeling reagent and hybridized with Affymetrix E.coli2.0 genome array chips.Data was analysed to get the effect of glycerol on E.coli gene expression

本研究探究了延长稳定期对大肠杆菌（Escherichia coli）全基因组表达的影响。综上，本研究结果表明，与新鲜培养的菌体相比，延长稳定期的大肠杆菌生长速率更低，这是由于翻译起始因子infC以及编码5S、16S、23S核糖体RNA的全部7个rrn操纵子的转录本丰度较低；这一情况进一步延缓了蛋白质合成进程，最终使得延长稳定期的大肠杆菌菌体生物量偏低。本研究将培养28天的大肠杆菌分别置于LB培养基以及添加10%体积分数甘油的LB培养基中培养。从上述样本中提取RNA并制备互补脱氧核糖核酸（cDNA），将片段化的cDNA通过标记试剂进行标记后，与Affymetrix E.coli2.0全基因组表达芯片进行杂交。对所得数据进行分析，以探究甘油对大肠杆菌基因表达的影响。