Data from: "Genome-wide microsatellite marker development from next-generation sequencing of two non-model bat species impacted by wind turbine mortality: Lasiurus borealis and L. cinereus (Vespertilionidae)" in Genomic Resources Notes accepted 1 October 2013 to 30 November 2013

Tree-roosting bats in the genus Lasiurus are widespread, migratory species that have not been well characterized for population genetic diversity and structure due to a lack of genetic resources. Generating genetic resources in Lasiurus is made pressing by the need for conservation genetic assessments of demographic trends in this genus, which comprise a large percentage of bat mortalities at wind turbine sites across North America. We report on marker development from whole-genome Illumina sequencing of the red bat (Lasirus borealis) and the hoary bat (L. cinereus). We generated paired-end libraries for a single individual of each species, sequenced on the Illumina HiSeq platform. We mapped a total of 46.6 million reads to the Myotis lucifigus reference genome, and used bioinformatics searches to identify tends of thousands of simple sequence repeats (SSRs) distributed across the bat genome. We selected 48 candidate microsatellite loci to develop cross-species primer sequences for Lasiurus, assembled these into multiplex combinations, and tested for amplification and polymorphism levels in a sample of 23 individuals from each of L. borealis and L. cinereus. In total, we identified 42 highly polymorphic loci that could be robustly amplified and scored, the majority of which (39) were also combinable into highly multiplexed assays of 4-8 loci each. The combination of new genomic sequence assemblies, a large set of highly polymorphic microsatellite loci, and the ability to efficiently multiplex represents a significant contribution to the genetic resources available for population and comparative genetic studies of bats.

拉斯乌拉属（Lasiurus）的树栖蝙蝠是一类分布广泛的迁徙性物种，由于遗传资源匮乏，其种群遗传多样性与种群结构尚未得到充分解析。该属物种在北美风电场导致的蝙蝠死亡中占比极高，因此对其种群动态开展保护遗传学评估的需求，使得构建拉斯乌拉属的遗传资源刻不容缓。本研究通过对红蝙蝠（Lasiurus borealis）与灰蝙蝠（L. cinereus）开展全基因组Illumina测序开发分子标记：我们为两个物种各选取1只个体构建双端测序文库，并利用Illumina HiSeq平台完成测序；将总计4660万条测序读段比对至小棕蝠（Myotis lucifugus）的参考基因组，通过生物信息学分析检索到分布于蝙蝠基因组的数万余个简单序列重复（simple sequence repeats, SSR）位点。我们从中筛选出48个候选微卫星位点，为拉斯乌拉属开发跨物种引物序列并组装为多重扩增组合，随后分别对23只红蝙蝠与23只灰蝙蝠样本开展扩增效果与多态性水平检测。最终共获得42个可稳定扩增并准确分型的高多态性位点，其中39个位点可进一步组合为每套包含4至8个位点的高效多重扩增体系。本研究新增的基因组序列组装结果、大量高多态性微卫星位点，以及高效的多重扩增体系构建能力，将为蝙蝠种群遗传学与比较遗传学研究提供更为丰富的遗传资源。