Cas9-induced large deletions and small indels are controlled in a convergent fashion

Demultiplexed reads assembled from 150PE Illumina using Pear with following settings: pear-0.9.10-bin-64 -f read1.fq -r read2.fq -n 20 -p 0.01. Unassembled PE reads (estimated at 0.1% of all reads) and reads that failed demux not included. 960 files total, corresponding to 96 wells of the library (95 control or knock-out mES clones and one well intentionally left empty) times two biological replicates times five test gRNAs (non-targetting control g33, chromosome X targetting g15, g48 and gU48 and autosome targetting g148). mES cells are derived from CAST x BL6 cross. Non-targeting control g33 fastq files are not locus-demultiplexed ie would map to g15, g48 and g148 loci.

本数据集包含经150bp双端Illumina测序得到的读段，通过Pear软件按以下参数组装得到的解多路复用（demultiplexed）序列：pear-0.9.10-bin-64 -f read1.fq -r read2.fq -n 20 -p 0.01。本数据集未包含未组装的双端读段（约占总读段的0.1%）以及解多路复用失败的读段。数据集总计包含960个文件，对应文库的96个孔位：其中95个为对照或敲除的小鼠胚胎干细胞（mouse embryonic stem cells, mES）克隆，1个孔位为空白对照；每个孔位对应2次生物学重复，每个重复对应5种向导RNA（guide RNA, gRNA）：非靶向对照g33、靶向X染色体的g15、g48与gU48，以及靶向常染色体的g148。所用mES细胞来源于CAST与BL6品系的杂交后代。非靶向对照g33的FASTQ文件未进行位点特异性解多路复用，即这些读段可比对至g15、g48及g148的靶位点。