Nuclear Protein Aggregates Disrupt RNA Processing and Alter Biomechanics in a Muscle Cell Model of OPMD

RNAsequencing in three subcellular fractions: cytosolic, nuclear and insoluble from vehicle-treated cells and Dox-treated cells that induces the expression of the expanded (A16) PABPN1. The goal of this study was to demonstrate PABPN1 function in A16 cells, and to identify RNA accummulation in each fraction. This allows the identification of molecular signatures. Overall design: Samples are from cytosolic, nuclear, and insoluble fractions isolated from vehicle and Dox-induced Ala16-PABPN1 in muscle cells. Biological replicates are N=3.

本数据集涵盖溶剂处理组细胞，以及可诱导表达扩增型(A16)多聚腺苷酸结合核蛋白1(PABPN1)的多西环素(Dox)处理组细胞的三类亚细胞组分的RNA测序数据，三类亚细胞组分为胞质组分、细胞核组分与不溶性组分。本研究旨在阐明A16细胞中PABPN1的功能，并鉴定各组分中的RNA积累情况，以此实现分子特征谱的识别。实验整体设计：样本取自肌细胞中经溶剂处理或Dox诱导表达Ala16-PABPN1的胞质、细胞核及不溶性组分，生物学重复数为n=3。