GSE47240: H3K9me1 (new lot).14-16 hr OR embryo.Solexa

Summary: modENCODE_submission_4123 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using the Illumina NGS sequencing platform. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: Embryo 14-16hr OR; Genotype: wild type; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Antibody H3K9me1 (new lot) (target is H3K9me1); Developmental Stage Embryo 14-16hr OR

摘要：modENCODE提交号4123
本提交来自Gary Karpen主导的modENCODE项目。如需查看modENCODE项目完整列表，请访问http://www.genome.gov/26524648。

项目目标：
本项目旨在解析黑腹果蝇（Drosophila melanogaster）全基因组范围内主要组蛋白修饰的定位分布。本次研究的修饰类型参与DNA复制、基因表达、基因沉默及遗传等核心染色体生物学过程。研究将采用Illumina下一代测序（NGS）平台开展染色质免疫共沉淀（Chromatin ImmunoPrecipitation，ChIP）实验。本研究将首先利用3种细胞系与2个胚胎时期的染色质进行修饰定位检测，后续还会将部分靶蛋白的分析拓展至另外4种动物组织/发育时期。

有关数据使用的条款与条件，请参阅http://www.genome.gov/27528022及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。

整体实验设计：
实验类型：ChIP-seq
生物样本来源：品系：Oregon-R（官方名称：Oregon-R-modENCODE，基因型：野生型）；发育时期：胚胎14-16小时（OR）；基因型：野生型
实验因素：品系Oregon-R（官方名称：Oregon-R-modENCODE，基因型：野生型）；抗体：H3K9me1（新批次，靶标为H3K9me1）；发育时期：胚胎14-16小时（OR）