Development of gene expression signatures for knock down expression of semaphorin 3F

To further development of our gene expression approach to biodosimetry, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish expression of semaphorin3F. Human colorectal cancer cell HCT116 was infected with empty control or eGFP-Sh-SEMA3F lentivirus,1468 genes was found altered significantly,709 genes was upregulated and 759 genes was downregulated significantly knock down expression of semaphorin3F induced gene expression in human colorectal cancer cell was measured. Three independent experiments were performed for each experiment

为推进我们应用于生物剂量测定（biodosimetry）的基因表达研究方法的发展，我们以全基因组微阵列表达谱分析（whole genome microarray expression profiling）作为发现平台，筛选可用于区分信号素3F（semaphorin3F）表达状态的基因。我们将空载体对照慢病毒（lentivirus）或携带增强绿色荧光蛋白标记的靶向SEMA3F短发夹RNA（eGFP-Sh-SEMA3F）慢病毒感染人结直肠癌细胞HCT116，经检测发现，敲低信号素3F的表达后，共有1468个基因的表达发生显著改变，其中709个基因表达上调、759个基因表达下调。本研究对敲低信号素3F表达后人结直肠癌细胞内的基因表达变化进行了测定。每项实验均设置三次独立重复实验。