Table_7_Early Diagnosis and Management of Nitrogen Deficiency in Plants Utilizing Raman Spectroscopy.pdf

Nutrient deficiency alters growth and development of crop plants and compromises yield. Real-time non-invasive monitoring of the nutritional status of crops would allow timely applications of fertilizers to optimize for growth and yield at different times of the plant’s life cycle. Here, we used Raman spectroscopy to characterize Arabidopsis and two varieties of leafy vegetable crops under nitrogen sufficient and deficient conditions. We showed that the 1046 cm–1 Raman peak serves as a specific signature of nitrogen status in planta, which can be used for early diagnosis of nitrogen deficiency in plants before onset of any visible symptoms. Our research can be applied toward crop management for sustainable and precision agriculture.

养分缺乏会改变作物的生长发育过程，并导致作物产量受损。对作物营养状况开展实时无损监测，可实现适时施肥，从而在作物生命周期的不同阶段优化其生长状态与产量表现。本研究采用拉曼光谱（Raman spectroscopy）技术，对氮充足及氮缺乏条件下的拟南芥（Arabidopsis）与两种叶类蔬菜作物进行了表征分析。研究结果表明，1046 cm⁻¹处的拉曼峰可作为植物体内氮素营养状况的特异性标志物，能够在作物出现任何可见症状之前，实现氮缺乏的早期诊断。本研究成果可应用于可持续农业与精准农业的作物管理实践中。