Phytochemical Characterization and Effect of Cagaita Leaf Extracts on Aspergillus Sp.

ABSTRACT Fungi of the genus Aspergillus may promote damage to the physiological quality of forest seeds, and plant extracts have been used to control microflora associated with these seeds. Thus, the aim of the present work was to perform the phytochemical characterization of ethanolic and aqueous extracts obtained from Eugenia dysenterica leaves (cagaita), as well as to evaluate the effect of these extracts on the development of Aspergillus sp. isolated from Hymenaea stigonocarpa seeds (jatobá-do-cerrado). For this, qualitative phytochemical analysis of extracts was carried out and their effect on Aspergillus sp mycelial growth, sporulation and spore germination was evaluated. As a result, phenols, hydrolysable tannins, flavonols, flavanones, xanthones, flavones, free steroids and saponins were found in both extracts, which could be used in the search for new antifungal compounds in the treatment of stored forest seeds, since extracts have secondary compounds that allow inhibiting Aspergillus sp mycelial growth.

摘要：曲霉属（Aspergillus）真菌可损害林木种子的生理品质，现有研究常采用植物提取物防控与这类种子相关的微生物群落。本研究旨在对卡盖塔（Eugenia dysenterica）叶片的乙醇提取物与水提取物开展植物化学成分表征，并评估这些提取物对分离自塞拉多雅托巴（Hymenaea stigonocarpa）种子的曲霉属真菌菌丝生长、产孢及孢子萌发的影响。据此，本研究对提取物开展了定性植物化学分析，并评估了其对该曲霉属真菌菌丝生长、产孢及孢子萌发的影响。结果显示，两种提取物中均检出酚类、水解单宁、黄酮醇、黄烷酮、呫吨酮、黄酮类、游离甾体及皂苷类化合物。鉴于此类提取物含有可抑制曲霉属真菌菌丝生长的次生代谢产物，因此可用于开发新型抗真菌化合物，以应用于仓储林木种子的病害防治。