Identification of DDX3X-dependent translation targets in the E11.5 mouse cortex

To identify DDX3X-dependent translation targets in the developing mouse cortex, we used E11.5 cortices from both sexes of wildtype and conditional Ddx3x knockout mice (driven by Emx1-Cre). We performed RNAseq and Riboseq on these samples in parallel. Overall design: Comparative gene expression profiling analysis of RNA-seq and Ribo-seq data for wildtype and Ddx3x conditional knockout mouse E11.5 cortex. The following are biological replicates: Female controls: F2, F3; Male controls: M1, M2, M3; cKO females: F1, F2, F3; cKO mals: M1, M2, M3

为鉴定发育中小鼠皮层中依赖于DDX3X（DDX3X）的翻译靶标，我们使用了由Emx1-Cre驱动的野生型与条件性Ddx3x敲除（conditional knockout, cKO）小鼠的雌雄两性胚胎11.5天（E11.5）皮层样本，并对这批样本并行开展了RNA测序（RNA-seq）与核糖体印记测序（Ribo-seq）实验。本研究的整体实验设计为：针对野生型与条件性Ddx3x敲除小鼠胚胎11.5天皮层的RNA-seq及Ribo-seq数据开展比较基因表达谱分析。生物学重复信息如下：雌性对照组：F2、F3；雄性对照组：M1、M2、M3；条件性敲除雌性组：F1、F2、F3；条件性敲除雄性组：M1、M2、M3。