Analysis of allogeneic and syngeneic CD4+ T cells reveals novel target genes and related pathway in acute graft-versus host disease

The goals of the study are to compare differently expressed genes in allogeneic and syngeneic CD4+ T cells isolated from splenocytes of allogeneic (donor: C57BL/6, host: Balb/c) and syngeneic (donor: Balb/c, host: Balb/c) HSCT mice. Overall design: RNA sequence was performed with allogeneic and syngeneic CD4+ T cells (n = 3 for each group) to identify the differently expressed genes and related pathway.

本研究旨在比较从同种异体（供体：C57BL/6，受体：Balb/c）与同基因（供体：Balb/c，受体：Balb/c）造血干细胞移植（HSCT）小鼠的脾细胞中分离得到的CD4阳性T细胞（CD4+ T cells）的差异表达基因。实验整体设计：对每组样本量均为3的同种异体与同基因CD4阳性T细胞进行RNA测序，以鉴定差异表达基因及其相关通路。