RNA sequencing reveals MUC5B-regulated downstream genes and pathways associated with lymph node metastasis in lung adenocarcinoma

This project presents RNA sequencing (RNA-seq) data generated to explore the molecular mechanisms by which MUC5B promotes lymph node metastasis in lung adenocarcinoma (LUAD). The datasets include transcriptomic profiles of two LUAD cell lines (A549 and H1975) subjected to MUC5B knockdown or overexpression, compared with their respective control groups.The primary aim of this sequencing is to identify differentially expressed genes and enriched signaling pathways downstream of MUC5B, with a focus on those involved in tumor invasiveness, metabolic reprogramming, and immune microenvironment regulation which are key processes underlying lymph node metastasis. These data provide a resource for investigating MUC5B-mediated regulatory networks and may facilitate the discovery of novel therapeutic targets for LUAD lymph node metastasis.

本项目呈现的RNA测序（RNA-seq）数据，旨在探究粘蛋白5B（MUC5B）促进肺腺癌（LUAD）淋巴结转移的分子机制。本数据集包含两株肺腺癌细胞系（A549与H1975）分别经MUC5B敲低或过表达处理后的转录组谱，并设置了对应的对照组进行比对。本次测序的核心目标为鉴定MUC5B下游的差异表达基因与富集信号通路，重点关注参与肿瘤侵袭、代谢重编程及免疫微环境调控的相关分子，上述过程均为淋巴结转移的关键致病环节。本数据集可为研究MUC5B介导的调控网络提供重要资源，亦有望助力肺腺癌淋巴结转移新型治疗靶点的发掘。