The transcriptional profile of neutrophils in different organs during pancreatic cancer

It was previously demonstrated that myeloid cells assume a distinct transcriptional profile when infiltrating the brain during chronic inflammation. We demonstrated that neutrophils infiltrate the brain during pancreatic cancer. To determine if the transcriptional profile of brain-infiltrating neutrophils is distinct from those infiltrating other organs, we compared the gene signatures of brain-infiltrating neutrophils, circulating neutrophils, liver-infiltrating neutrophils, and tumor-infiltrating neutrophils to that of circulating neutrophils from healthy mice. Overall design: Brain-infiltrating neutrophils (n=4), Liver-infiltrating neutrophils (n=4), Tumor-infiltrating neutrophils (n=4), Circulating neutrophils (from tumor-bearing mice) (n=4), Circulating neutrophils (from sham mice) (n=4)

既往研究已证实，髓系细胞在慢性炎症过程中浸润大脑时，会呈现独特的转录谱。本研究证实，胰腺癌发生时中性粒细胞会浸润大脑。为明确大脑浸润性中性粒细胞的转录谱是否与其他器官浸润的中性粒细胞存在差异，我们将大脑浸润性中性粒细胞、循环中性粒细胞、肝脏浸润性中性粒细胞及肿瘤浸润性中性粒细胞的基因特征，与健康小鼠循环中性粒细胞的基因特征进行了比对分析。实验设计概述：大脑浸润性中性粒细胞（n=4）、肝脏浸润性中性粒细胞（n=4）、肿瘤浸润性中性粒细胞（n=4）、荷瘤小鼠循环中性粒细胞（n=4）、假手术小鼠循环中性粒细胞（n=4）