Transcription analysis of E. coli O103:H25 in co-culture with Bacteroides thetaiotaomicron CCUG 10774

Investigation of whole genome gene expression level changes in E. coli O103:H25 in co-culture with Bacteroides thetaiotaomicron CCUG 10774 compared to when E. coli is cultured individually. A study using total RNA recovered from three separate co-cultures (E. coli O103:H25 and B. thetaiotaomicron 1:100) at OD=0.5, compared to when E. coli is cultured individually (5 separate samples including 1-3 technical replicates of each sample). Three separate samples are also recovered when E. coli is cultured in supernatant from Bacteroides thetaiotaomicron at OD=0.5 compared to when E coli is cultured alone. A comparison is also done after treatment with Mitomycin C (MMC). MMC is added at OD=0.5. There are 3 separate samples from each group that are compared (co-culture versus E. coli grown individually, E. coli in supernatant from B. thetaiotaomicron versus E. coli grown individually).

本研究旨在探究大肠埃希菌O103:H25（E. coli O103:H25）与多形拟杆菌（Bacteroides thetaiotaomicron）CCUG 10774共培养时，其全基因组基因表达水平相较于单培养状态的变化。本研究通过以下三组实验开展对比分析：第一组为共培养对照实验，提取3组独立共培养体系（大肠埃希菌O103:H25与多形拟杆菌接种比例为1:100，培养至光密度OD=0.5时收集）的总RNA，以单培养大肠埃希菌为对照，对照组共设置5组独立样本，每组包含1-3次技术重复；第二组为上清培养对照实验，收集大肠埃希菌在多形拟杆菌上清液中培养至OD=0.5时的总RNA，以单培养大肠埃希菌为对照，共设置3组独立样本；第三组为丝裂霉素C（Mitomycin C, MMC）处理实验，当培养至OD=0.5时加入MMC，每组均设置3组独立样本，对比内容包含共培养与单培养大肠埃希菌、多形拟杆菌上清液培养与单培养大肠埃希菌的基因表达差异。