The miR-183/96/182 cluster is a checkpoint for resident immune cells and shapes the cellular landscape of the cornea.

The conserved miR-183/96/182 cluster (miR-183C) regulates both corneal sensory innervation and corneal resident immune cells (CRICs). This study is to uncover its role in CRICs and in shaping the corneal cellular landscape at a single-cell (sc) level. Corneas of naive, young adult [2 and 6 months old (mo)], female miR-183C knockout (KO) mice and wild-type (WT) littermates were harvested and dissociated into single cells. Dead cells were removed using a Dead Cell Removal kit. CD45 + CRICs were enriched by Magnetic Activated Cell Sorting (MACS). scRNA libraries were constructed and sequenced followed by comprehensive bioinformatic analyses保守型miR-183/96/182基因簇（miR-183C）可同时调控角膜感觉神经支配与角膜驻留免疫细胞（CRICs）。本研究旨在阐明该基因簇在CRICs中的功能，并从单细胞（sc）水平解析其对角膜细胞图谱的塑造机制。实验采集了未致敏年轻成年（2月龄与6月龄）雌性miR-183C敲除（KO）小鼠及其野生型（WT）同窝对照的角膜组织，将其解离为单细胞悬液；采用死细胞去除试剂盒清除死细胞，通过磁激活细胞分选（MACS）富集CD45阳性CRICs；随后构建单细胞RNA测序文库并完成测序，进而开展全面的生物信息学分析。