Cytokine Signaling Co-opts the CREB/CRTC Pathway in Obesity

Cooperative effects of CREB and NF-kB Pathways on Adipose Tissue Inflammation in Obesity Overall design: epididymal WAT from normal chow or 15wks high fat diet fed mice and WT_IgG or CRTC2/3 dAKO_IgG or WT_CXCL1/2 neutralizing Ab injected mice under 9wks high fat feeding

环腺苷酸应答元件结合蛋白（CREB）与核因子κB（NF-kB）通路在肥胖状态下对脂肪组织炎症的协同效应
实验整体设计：采集普通饲料喂养小鼠、高脂饲料喂养15周小鼠的附睾白色脂肪组织（epididymal WAT）；同时采集经9周高脂饲料喂养后，分别注射免疫球蛋白G（Immunoglobulin G，IgG）对照的野生型小鼠、免疫球蛋白G（Immunoglobulin G，IgG）对照的CRTC2/3双敲除（double knockout，dAKO）小鼠，以及注射CXCL1/2中和抗体（neutralizing antibody）的野生型小鼠的附睾白色脂肪组织。