Manganese detoxification by Staphylococcus aureus. Manganese detoxification by Staphylococcus aureus

Manganese (Mn) is an essential micronutrient critical for the pathogenesis of Staphylococcus aureus, a significant cause of human morbidity and mortality. Paradoxically, excess Mn is toxic, therefore maintaining intracellular Mn homeostasis is required for survival. To identify gene candidates that contribute to manganese detoxification, we compared the transcriptional response of S. aureus cells exposed to 1 mM MnCl2 and those that were untreated. Overall design: Cultures of S. aureus were exposed to either 1 mM MnCl2 or untreated media. The experiment was performed in biological triplicate for a total of 6 samples.

锰（Manganese, Mn）是一种必需微量营养素，其与金黄色葡萄球菌（Staphylococcus aureus）的致病过程紧密相关，而该菌正是引发人类发病与死亡的重要病原菌。矛盾的是，过量的锰会产生毒性，因此维持细胞内锰稳态是该菌存活的必要条件。为筛选参与锰解毒的候选基因，本研究对比了暴露于1 mM氯化锰（MnCl2）的金黄色葡萄球菌细胞与未处理对照组的转录应答。实验设计：将金黄色葡萄球菌培养液分别暴露于1 mM MnCl2或未添加处理的培养基中，本实验设置三次生物学重复，总计6个样本。