Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - m3c25_h1930002_CMN. Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - m3c25_h1930002_CMN

Illustrating the cellular architecture of the human brain is critical to understanding its diverse functions and complex animal behaviors. Single nucleus methylation sequencing was applied to regions of human brains. we identified distinct cell clusters and formed them in a hierarchical taxonomy. These clusters include known primary brain cell types and possible sub-types. We use these data to identify the epigenomic characteristics and to define specific regulatory elements for each cell cluster. Overall design: Apply snm3C-seq to brain regions to identify epigenomic landcape of the adult human brains

阐明人类大脑的细胞架构，是解析其多样功能与复杂动物行为的关键所在。本研究对人类大脑多脑区开展单细胞核甲基化测序（Single nucleus methylation sequencing），鉴定得到多组独立细胞簇，并构建了层级分类体系。这些细胞簇既包含已报道的主要脑细胞类型，也涵盖潜在的亚型。本研究利用该数据集解析了各组细胞簇的表观基因组特征，并明确了每类细胞的特异性调控元件。整体实验设计：对脑区实施snm3C-seq测序，以解析成年人类大脑的表观基因组图谱。