mRNA sequencing of Trim71 conditional ("WT") and Trim71 KO mES cells. Mus musculus

We report that Trim71 mutation does not lead to significant changes within the core regulatory network of mES cells, however, there is an upregulation of specific genes involved in neural differentiation. We thus conclude that Trim71 KO mES cells are poised for differentiation. Overall design: Analysis of changes in the expression of mRNAs in two different genotypes

本研究发现，Trim71突变不会导致小鼠胚胎干细胞（mouse embryonic stem cells, mES cells）的核心调控网络发生显著改变，但会使参与神经分化的特定基因出现表达上调。据此我们得出结论：Trim71敲除小鼠胚胎干细胞处于分化就绪状态。
实验整体设计：对两种不同基因型样本的信使RNA（messenger RNA, mRNA）表达变化展开分析。