Genetics of the black-lipped pearl oyster, Pinctada margaritifera in the western Pacific

Seven populations of the black-lipped pearl oyster, Pinctada margaritifera, from the western Pacific were assayed for allozyme variation at 17 polymorphic loci from mantle biopsies.Gene frequencies were able to be reliably scored on 7 polymorphic loci: EST, LT-1, LT-2, LP, PGM, MDH-1, GPI. Numbers of samples (total 268) ranged from 5 to 98 per collection locality.Genetic relationship was examined using Rogers genetic distance, Nei's unbiased genetic distances and UPGMA clustering method.Collection localities: Batt and Little Trunk reefs on the Great Barrier Reef; Butatitari and Abaiang in Kiribati; and Manihiki, Penryhn and Suwarrow in the Cook Islands. To examine the genetic differentiation between populations within reef groups and additionally between the Great Barrier Reef and Pacific Islands.

本研究对西太平洋7个黑唇珍珠贝（Pinctada margaritifera）种群的外套膜活检样本进行了17个多态性位点的等位酶变异分析。其中7个多态性位点（EST、LT-1、LT-2、LP、PGM、MDH-1、GPI）的基因频率可被可靠测定。各采集地点的样本数量总计268个，单地点样本量介于5至98个之间。研究采用罗杰斯遗传距离、Nei无偏遗传距离及UPGMA聚类法分析种群间的遗传关系。采集地点包括：大堡礁的巴特礁与小特兰克礁；基里巴斯的布塔蒂塔里岛与阿拜昂岛；以及库克群岛的马尼希基岛、彭林岛与苏瓦罗岛。本研究旨在探究礁群内种群间的遗传分化，以及大堡礁与太平洋岛屿种群间的遗传分化。