Integrated Paired-end Enhancer Profiling and Whole-Genome Sequencing Reveals Recurrent CCNE1 and IGF2 Enhancer Hijacking in Primary Gastric Adenocarcinoma [CapStarr-seq]. Integrated Paired-end Enhancer Profiling and Whole-Genome Sequencing Reveals Recurrent CCNE1 and IGF2 Enhancer Hijacking in Primary Gastric Adenocarcinoma [CapStarr-seq]

H3K27ac paired-end NanoChIP-seq, whole-genome sequecing, RNA-seq and Hi-C were integrated to reveal tumor-associated structural variants contributing to gastric cancer Overall design: H3K27ac paired-end NanoChIP-seq were generated from primary GC and matched normal samples to discover regulatory structural variations that occurred at cis-regulatory elements marking by H3K27ac mark.

本研究通过整合组蛋白H3赖氨酸27乙酰化（H3K27ac）双端NanoChIP-seq、全基因组测序（whole-genome sequencing）、RNA测序（RNA-seq）以及Hi-C技术，以揭示与胃癌相关的致病结构变异。总体实验设计如下：从原发性胃癌（Gastric Cancer, GC）及配对正常组织样本中制备实验材料，构建组蛋白H3赖氨酸27乙酰化双端NanoChIP-seq文库并完成测序，旨在发掘位于受H3K27ac标记的顺式调控元件上的调控性结构变异。