Amplification of DNA Brownian Motion Using Electrokinetic Noise

This dataset was collected to study the effects of applied white Gaussian electrokinetic noise on the diffusivity of single DNA molecules inside a nanofluidic slit. Our raw data consists of image sequences in FITS format (Flexible Image Transport System). Each image shows a fluorescent Lambda DNA molecule stained by YOYO-1 dye at a 10:1 base-pair to dye ratio diffusing inside a 110 nm deep nanoslit under specified amounts of applied noise. An imaging system comprising an inverted Nikon TE2000U microscope, an ANDOR iXon 897 EMCCD camera, an X-Cite UV illumination system, and a 60X, 0.7 NA, water-immersion objective lens with an extra 1.5X magnification factor was used to acquire the images. We applied 8 different noise intensities and recorded about 50-70 molecules per noise intensity. These data can be used to find trajectories of the centers of mass of the molecules and to analyze the radii of gyration and DNA shapes. Sigma represents the standard deviation of the applied Gaussian noise.

本数据集旨在探究外加白高斯电动噪声对纳米流体狭缝内单DNA分子扩散率的影响。原始数据为FITS（Flexible Image Transport System）格式的图像序列。每张图像均展示了以碱基对与染料比例10:1的YOYO-1染料染色的荧光Lambda DNA分子，在深度为110 nm的纳米狭缝中，于特定强度的外加噪声环境下扩散的场景。图像采集采用的成像系统包括倒置尼康TE2000U显微镜、ANDOR iXon 897 EMCCD相机、X-Cite紫外照明系统，以及带有1.5倍额外放大倍率的60倍、0.7数值孔径水浸物镜。本研究设置了8种不同的噪声强度，每种强度下记录约50至70个分子的观测数据。该数据集可用于提取分子质心轨迹，分析分子的回转半径与DNA形态。Sigma代表所施加高斯噪声的标准差。