Hand eczema and changes in the skin microbiome after two weeks of topical corticosteroid treatment

Analysis of skin swabs collected from adult patients (N=35) with hand eczema (HE) in Denmark. Samples were collected from lesional and nonlesional skin of the hand before (visit 1) and after (visit 2) two weeks of topical corticosteroid (TCS) treatment. Amplicon sequencing of the v3v4 region of the 16S rRNA gene were performed in order to investigate treatment related changes in the cutanous bacterial. communities.

本研究针对丹麦地区35名成年手部湿疹（hand eczema, HE）患者的皮肤拭子样本开展分析。研究人员于局部糖皮质激素（topical corticosteroid, TCS）治疗两周前（访视1）及治疗两周后（访视2），分别采集了患者手部皮损区与非皮损区的样本。为探究治疗相关的皮肤细菌群落变化，本研究对16S rRNA基因（16S rRNA gene）的v3v4可变区进行了扩增子测序（amplicon sequencing）。