Next Generation Sequencing of ileum in broiler infected with S. pullorum

This study was conducted to evaluate the effects of dietary supplemental magnolol and honokiol in broilers infected with S. pullorum. A total of 360 one-day-old broilers were selected and randomly divided into four groups with six replicates: the negative control group (CTL), S. pullorum-infected group (SP), and the S. pullorum-infected group supplemented with 300 mg/kg honokiol (SPH) or magnolol (SPM). Chicks in the SP, SPH, and SPM groups were orally treated with a 0.5 ml (4×108 CFU/mL) S. pullorum solution at 5 days of age, while chicks in the control (CTL) group received the same amount of sterilized PBS at the same age.At 14 and 21 days of age, one chick from each replicate was randomly selected to be weighed and slaughtered by jugular exsanguination after a 12-h fasting period. The ileum samples were collected to analyze the differential expression genes. Examination of gene in ileum microbiota of broiler infected with S. pullorum with or without magnolol and honokiol supplementation.

本研究旨在评估日粮添加厚朴酚（magnolol）与和厚朴酚（honokiol）对感染鸡白痢沙门氏菌（Salmonella Pullorum，S. pullorum）肉鸡的影响。共计选取360只1日龄肉鸡，随机分为4组，每组设置6个重复：阴性对照组（CTL）、鸡白痢沙门氏菌感染组（SP），以及分别添加300 mg/kg和厚朴酚（SPH）、厚朴酚（SPM）的鸡白痢沙门氏菌感染组。SP、SPH及SPM组雏鸡于5日龄时经口灌服0.5 mL浓度为4×10^8 CFU/mL的鸡白痢沙门氏菌菌液，CTL组雏鸡于同日龄灌服等量无菌磷酸盐缓冲液（PBS）。于14日龄与21日龄时，每个重复随机选取1只雏鸡，经12 h禁食后采用颈静脉放血法处死并称量体重，采集回肠样本以分析差异表达基因。本研究同时对感染或未感染鸡白痢沙门氏菌，且分别添加或不添加厚朴酚与和厚朴酚的肉鸡回肠微生物群相关基因进行检测分析。