[3H]S107 binding to SR vesicles.

SR vesicles with low and high Bmax of [3H]ryanodine binding were obtained as described in Materials and Methods. Bmax of [3H]ryanodine binding was obtained as shown in Fig. S2A. Shown are the amounts of [3H]ryanodine specifically bound to SR vesicles after 5 h at 24°C. Specific [3H]S107 binding to SR vesicles was determined at 44 µM [3H]S107 and indicated free Ca2+ concentration as described in Fig. S2B. Shown are the amounts of S107 specifically bound to SR vesicles after 9 h incubation at 24°C. Data are the mean ± SD of 3–6 determinations.

按照材料与方法所述，我们制备得到具有低、高[³H]雷诺丁碱（[3H]ryanodine）结合最大结合容量（Bmax）的肌浆网囊泡（SR vesicles）。[³H]雷诺丁碱结合的最大结合容量测定结果如图S2A所示。本结果展示了24℃孵育5小时后，特异性结合于肌浆网囊泡的[³H]雷诺丁碱含量。以44 μM的[³H]S107为探针测定肌浆网囊泡的特异性[³H]S107结合，并如图S2B所述标注了游离钙离子浓度。本结果展示了24℃孵育9小时后，特异性结合于肌浆网囊泡的S107含量。数据为3~6次独立测定的平均值±标准差。