Negative air ion generator as air spora Remover in Poultry farm.

The present study was to determine the fungal spores count in the absence and presence of negative air ion (NAIs) generator in a poultry farm at Kolhapur (16.70oN, 74.24oE), India. We installed the NAI generator to generate artificially negative ion continuously in the poultry farm. Fungal spores were collected for a period of one month in December 2019 by using Volumetric Tilak Air Sampler. A total of 10690 spores counted of which 6460 number of fungal spores collected in absence while 4210 number of spores collected in the presence of the NAI generator from the poultry farm. The 16 different types of fungal spores recorded of which Aspergillus, Smut, and Alternaria spore type observed as most dominant in both the absence and presence of NAIs. The qualitative analysis showed a total of 16 different fungal spores types were identified in which Aspergillus, Alternaria, Nigrospora, Tourla are reported human pathogens. Among the total number of isolated fungal spores in the absence of NAI in the poultry farm Aspergillus (1285), Alternaria (1063), Smut (900), Nigrospora (472), Cladosporium (106) types of spores were observed as most dominant fungal spores but Hyphalfragment (0), Leptosphaeria (15) were least recorded Whereas in the presence of NAI in the poultry farm the dominant fungal spores counted were also Aspergillus (1242), Alternaria (511), Smut (501), Nigrospora (296) and Hyphalfragment (0), Leptosphaeria (24). In our study the hypothesis is the Negative air ion attached themselves to particles such as dust, mold spores, and other allergens . Negative air ions are lightweight as compare to positive air ion in the poultry farm. The mass of negative air ion is low. Therefore, the mobility of negative air ion is high. These high mobility negative air ions attached to the fungal spores in the poultry farm and settle down on the surface. Most of the spores carry positively charged which affects the health of birds, workers work in the poultry farm. Due to the installation of the NAI generator in the poultry farm which generates negative air ion in the poultry farm. These NAI should get attracted to the fungal spores having positively charged and it get neutral or combining these negative air ions with spores the weight of fungal spores increased in the air and it gets to settle down to the surface.

本研究旨在测定印度科尔哈普尔（16.70°N，74.24°E）某养鸡场在有无负空气离子（negative air ion, NAI）发生器的情况下的真菌孢子计数。我们在该养鸡场安装了负空气离子发生器，以持续人工生成负空气离子。2019年12月，我们采用体积式蒂拉克空气采样器（Volumetric Tilak Air Sampler）开展了为期1个月的真菌孢子采集工作。本次研究共计数真菌孢子10690个，其中无负空气离子发生器时采集到6460个，配备该发生器时采集到4210个。共记录到16种不同的真菌孢子，其中曲霉属（Aspergillus）、黑粉菌（Smut）与链格孢属（Alternaria）孢子在有无负空气离子发生器的场景下均为优势孢子类型。
定性分析共鉴定出16种真菌孢子，其中曲霉属、链格孢属、黑孢霉属（Nigrospora）、图拉孢属（Tourla）被报道为人类致病菌。在无负空气离子发生器的养鸡场中，分离得到的优势真菌孢子为曲霉属（1285个）、链格孢属（1063个）、黑粉菌（900个）、黑孢霉属（472个）与枝孢属（Cladosporium，106个）；菌丝片段（Hyphalfragment，0个）、小球腔菌属（Leptosphaeria，15个）为检出量最低的孢子类型。在配备负空气离子发生器的养鸡场中，优势真菌孢子依次为曲霉属（1242个）、链格孢属（511个）、黑粉菌（501个）、黑孢霉属（296个），检出量最低的仍为菌丝片段（0个）与小球腔菌属（24个）。
本研究的假说为：负空气离子可附着于粉尘、霉菌孢子及其他过敏原颗粒。养鸡场内的负空气离子相较于正空气离子质量更轻，因此迁移性更强。这类高迁移性的负空气离子可与养鸡场内的真菌孢子结合并沉降至物体表面。多数真菌孢子携带正电荷，会对养鸡场禽类与工作人员的健康产生不利影响。在养鸡场安装负空气离子发生器后，其所生成的负空气离子可与带正电的真菌孢子结合，使孢子电荷得到中和；同时，结合后的真菌孢子在空气中的重量增加，最终沉降至物体表面。