Gene expression profile for BL2 and BL2 AID-/- cell lines. Homo sapiens

AID (activation-induced cytidine deaminase) is expressed at low levels in many tissue types but is most highly expressed in germinal center B cells where it deaminates cytidine to uracil during somatic hypermutation and class switch recombination of the immunoglobulin genes. In addition to this critical role in immune diversification, aberrant targeting of AID contributes to oncogenic point mutations and chromosome translocations associated with B cell malignancies. Thus, to explore a role for AID in DNA demethylation in B cell lymphoma, we performed genome-wide gene expression profiling in BL2 and AID-deficient (AID-/-) BL2 cell lines (Burkitt lymphoma that can be induced to express high levels of AID). Overall design: The Human4$4 targets more than 47,000 probes derived from the National Center for Biotechnology Information Reference Sequence (NCBI) RefSeq Release 38 (November 7, 2009) and other sources. We did it in two samples BL2 and BL2 AID-/- cell lines.

活化诱导的胞苷脱氨酶（activation-induced cytidine deaminase，AID）在多种组织中呈低水平表达，但在生发中心B细胞中表达量最高；该酶可在免疫球蛋白基因的体细胞超突变与类别转换重组过程中，将胞嘧啶脱氨为尿嘧啶。除了在免疫多样化过程中发挥关键作用外，AID的异常靶向还会促成与B细胞恶性肿瘤相关的致癌点突变与染色体易位。为探究AID在B细胞淋巴瘤DNA去甲基化中的作用，我们在BL2细胞系及AID缺陷型（AID-/-）BL2细胞系（可诱导高表达AID的伯基特淋巴瘤细胞系）中开展了全基因组基因表达谱分析。整体实验设计：Human4$4芯片包含超过47000个探针，这些探针源自美国国家生物技术信息中心参考序列（National Center for Biotechnology Information Reference Sequence，NCBI RefSeq）第38版（2009年11月7日）及其他相关来源。本实验共设置两个样本组：BL2细胞系与BL2 AID-/-细胞系。