Additional file 3 of Quantitative microbial population study reveals geographical differences in bacterial symbionts of Ixodes ricinus

Additional file 3: Table S4. Read counts for different taxa, including taxonomy per sample. Negative control samples included. Figure S1. Ordination of community composition with uncorrected abundances. Figure S2. Rarefaction curve of samples coloured by quartile of total bacterial load. Figure S3. Abundances of bacterial phyla separated by quartiles of total bacterial load (16S rRNA content in ng/μL), unscaled (a) or scaled by total bacterial load (b). Table S5. Figure S4. Correlation of Shannon diversity of the unrarefied data to (a) sequencing depth and (b) total bacterial load. Figure S5. Correlation of total bacterial load to (a) sequencing depth and (b) uncorrected count of contaminant sequences. Figure S6. The top 10 genera of negative controls versus the 25% lowest biomass samples.

附加文件3：表S4。不同分类群的读长计数，含各样本的分类学信息，纳入阴性对照样本。图S1：未校正丰度下的群落组成排序分析。图S2：按总细菌载量四分位数分组着色的样本稀疏曲线。图S3：按总细菌载量（16S rRNA含量，单位ng/μL）四分位数分组的细菌门丰度，分别展示未标准化（a）与按总细菌载量标准化（b）的结果。表S5。图S4：未稀疏化数据的香农多样性（Shannon diversity）与（a）测序深度、（b）总细菌载量的相关性分析。图S5：总细菌载量与（a）测序深度、（b）未校正的污染序列计数的相关性分析。图S6：阴性对照样本的前10个菌属与生物量最低的25%样本的菌属对比。