Regulatory Mechanism of Systemic Adaptation in Heat-Stressed Dairy Cows Based on RNAseq Analysis

Heat stress can cause a series of endocrine changes, metabolic remodeling and immune imbalance. However, it is difficult to systematically elucidate the interaction mechanisms and nutrient repartitionpathways of different tissues and organs under heat stress based on research methods based on conventional blood indicators or single tissue transcriptomes. The team's previous studies have shown that heat stress can cause inflammatory responses in breast tissue, but its underlying causes and specific mechanisms still need further exploration. Therefore, this study combined RNAseq, arteriovenous differential analysis, and single-cell sequencing to conduct the following research on the metabolic adaptation of multiple tissues of dairy cows under heat stress, immune response characteristics, and the effects of mammary nutrient substrates and hormone regulation patterns on lactation performance

热应激可引发一系列内分泌改变、代谢重塑与免疫失衡。然而，基于传统血液指标或单组织转录组学的研究方法，难以系统阐明热应激状态下不同组织器官间的互作机制与养分再分配通路。本团队前期研究表明，热应激可引发乳腺组织炎症反应，但其潜在诱因与具体调控机制仍有待进一步探究。因此，本研究联合RNA测序（RNAseq）、动静脉差异分析与单细胞测序技术，针对热应激条件下奶牛多组织的代谢适应机制、免疫应答特征，以及乳腺营养底物与激素调控模式对泌乳性能的影响开展相关研究。