CRM image scan of 6 day old biofilm, cosmic ray corrected, exported in ASCII format

WiTec Project file of a CRM image scan of the 6 day old Shewanella oneidensis biofilm. From M&M: The measurement was performed on a WITec alpha300 confocal Raman microscope, using a 50 µm pinhole and a Zeiss LD plan-NEOFLUAR 20×/0.4 corr air objective with coverslip correction. 20× magnification was used in order to keep as many bacteria as possible in the focal plane while still resolving individual bacteria. The excitation wavelength was 532 nm, with laser power of 3 mW at the focal plane. The Raman detector was a newton EMCCD camera cooled to -60o C with a 600 g/mm grating. Integration time was 1 second and lateral resolution 2 pixel/µm. The file was exported in ASCII format.

本数据集为针对6日龄奥奈达希瓦氏菌（Shewanella oneidensis）生物膜的共聚焦拉曼（Confocal Raman Microscopy, CRM）成像扫描的威泰克（WiTec）项目文件。数据来源标注为M&M：本次测量采用威泰克alpha300型共聚焦拉曼显微镜完成，配置50 µm针孔，搭配蔡司（Zeiss）LD plan-NEOFLUAR 20×/0.4 corr air物镜，具备盖玻片校正功能。选用20倍放大倍率，以尽可能将更多细菌保留在焦平面内，同时仍可分辨单个细菌。激发波长为532 nm，焦平面处激光功率为3 mW。拉曼探测器为冷却至-60℃的牛顿（Newton）EMCCD相机，搭载600 g/mm光栅。积分时长为1秒，横向分辨率为2像素/微米。该文件以ASCII格式导出。