Brain microvessel-specific transcriptome profiling after severe cerebral hypoperfusion in mice

Little is understood about the underlying cellular and molecular mechanisms related to brain microvessel damage and glial activation after severe cerebral hypoperfusion. Efforts to explore the relationship between neuropathological and gene expression changes support a role for identifying novel molecular pathways by transcriptomic mechanisms. To investigate brain microvessel-specific transcriptome profiling after severe cerebral hypoperfusion in mice Brain microvessel-specific gene expression profiling analysis was performed using data obtained from RNA-seq of 3 biological replicates in sham and BCAS mice after hypoperfusion

目前针对重度脑低灌注后引发的脑微血管损伤与胶质细胞激活相关的潜在细胞及分子机制，学界尚缺乏系统深入的认知。现有针对神经病理变化与基因表达改变之间关联的探索性研究，证明了借助转录组学技术识别新型分子通路的重要意义。为探究小鼠重度脑低灌注后脑微血管特异性转录组表达谱，本研究针对假手术（sham）组与双侧颈总动脉狭窄（BCAS）小鼠低灌注后的样本，采用3次生物学重复的RNA测序（RNA-seq）数据，开展了脑微血管特异性基因表达谱分析。