HEK293 stably expressing Autoimmune regulator protein. Homo sapiens

Auto Immune REgulator (AIRE) protein expression in HEK293 cells Overall design: Three cell-lines where used in the study: HEK293-NC; control cell-line expressing yellow fluorescent protein (YFP) HEK-AIRE1, HEK-AIRE2; two different clone-derived Autoimmune regulator protein expressing cell-lines HEK-AIRE-D312A; cell line expressing Autoimmune regulator protein with D312A mutation in the first PHD finger With all cell-lines three independent experiments were performed.

HEK293细胞中自身免疫调节因子（Auto Immune REgulator, AIRE）的蛋白表达分析
实验设计概述：本研究共纳入3种细胞系：HEK293-NC，即表达黄色荧光蛋白（yellow fluorescent protein, YFP）的阴性对照细胞系；HEK-AIRE1与HEK-AIRE2，两种由不同单克隆株衍生的自身免疫调节因子蛋白表达细胞系；HEK-AIRE-D312A，在首个PHD锌指结构域（PHD finger）携带D312A突变的自身免疫调节因子蛋白表达细胞系。所有细胞系均完成3次独立重复实验。